One-step PCR Clean-up for optimal sequencing results. Clean-up results in 5 minutes.
FEATURES
>One-step PCR product clean up
>Fast 5 min protocol
>No Sample Loss
>No need for spin columns or magnetic beads
>Treatment improves downstream applications such as DNA sequencing and SNP analysis
>Ideal for NGS library preparation protocols
DESCRIPTION
PureIT ExoZAP PCR CleanUp consists of a balanced combination of a heat labile Exonuclease (HL-ExoI) I and a recombinant Shrimp Alkaline Phosphatase (rSAP).
Treatment of amplified PCR products with PureIT ExoZAP PCR CleanUp, removes residual primers, single-stranded DNA and inactivates excess dNTPs by dephosphorylation. Add PureIT ExoZAP PCR CleanUp directly to the reaction containing the amplified PCR product. After treatment at 37 °C for minimum 2 minutes, PureIT ExoZAP PCR CleanUp is completely inactivated by heating at 80 °C for minimum 3 minutes.