General notes
Pre-cut membranes (10 sheets, 7 x 9 cm), compatible with gels 10 x 10 cm and smaller. Note: PVDF membranes must be activated prior to use. Membrane activation protocol: 1. Pre-wet the membrane in methanol for a few moments until the membrane is completely wet. 2. Transfer the wet membrane from methanol to purified water and incubate on a rocker or shaker for at least 5 minutes. Make sure that the membrane is fully immersed in water and does not float on the water’s surface. 3. Transfer the membrane from water to transfer buffer, and incubate for 5 minutes.
