Optimum solution for the acid/basic hydrolysis of food and feed samples prior to solvent extraction for total fat analysis.
Preliminary Hydrolysis for solvent extraction
The VELP Scientifica Hydrolysis Unit HU 6 is the ideal solution for the prior to solvent extraction for the determination of the total fat content, in order to free the fat molecules ready for extraction.
This procedure is required in a number of AOAC Official Methods to determine the fat content of food samples such as meat, cheese, seafood, chocolate, cereal flours, etc.
The HU 6 performs hydrolysis in complete safety and handles six samples at the same time in order to maximize productivity.
Fast and safe hydrolysis
The samples are heated in test-tubes in an aluminum heating block offering excellent thermal homogeneity. Hydrolysis is performed in an acidic environment (4N hydrochloric acid) and in some cases also in a basic environment (20% w/v ammonia) for about 60 minutes at a temperature of 170°C. The hydrolyzed sample is then filtered in a glass crucible and washed with warm deionized water to eliminate traces of hydrochloric acid.
The HU 6 is supplied with a vacuum pump that ensure safety thanks to the reduction of acid fumes.
The glass crucible containing the hydrolyzed and washed sample is oven dried before being placed in a desiccator. The crucible is then transferred directly to the SER 158 and SER 148 Solvent Extraction Unit avoiding any possible sample loss and ensuring improved accuracy of results.