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HomeLife ScienceProteomicsWestern Blot (HRP) Goat Anti RAbbit & Mouse
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(HRP) Goat Anti RAbbit & Mouse

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SKU: N/A Categories: Elisa, Immunohistochemistry (IHC) / Immunofluorescence (IF), Life Science, Western Blot Tag: abcam
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  • Description
  • Item List
  • Properties Goat Anti-Rabbit
  • Applications Goat Anti-Rabbit
  • Properties Goat Anti-Mouse
  • Applications Goat Anti-Mouse
  • Datasheet
Description

Goat Anti-Rabbit IgG H&L (HRP)

  • Product name

    Goat Anti-Rabbit IgG H&L (HRP)
    See all IgG secondary antibodies
  • Host species

    Goat
  • Target species

    Rabbit
  • Tested applications

    Suitable for: IHC-P, WB, ELISA, Immunomicroscopy, Dot blot, ICC, IHC-Frmore details
  • Immunogen

    Rabbit IgG, whole molecule

  • Conjugation

    HRP

 

Goat Anti-Mouse IgG H&L (HRP)

  • Product name

    Goat Anti-Mouse IgG H&L (HRP)
    See all IgG secondary antibodies
  • Host species

    Goat
  • Target species

    Mouse
  • Tested applications

    Suitable for: ICC, IP, Dot blot, ELISA, IHC-P, IHC-Fr, Immunomicroscopy, WBmore details
  • Immunogen

    mouse IgG whole molecule

  • Conjugation

    HRP
Item List

Item List

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Properties Goat Anti-Rabbit

Properties Goat Anti-Rabbit

Goat Anti-Rabbit IgG H&L (HRP)

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. Please see notes section. Store In the Dark.
  • Storage buffer

    Preservative: 0.01% Gentamicin sulphate
    Constituents: 0.42% Potassium phosphate, 0.88% Sodium chloride
  • Concentration
    • 1 mg at 2 mg/ml

     

     

  • Purity

    Immunogen affinity purified
  • Purification notes

    This product was prepared from monospecific antiserum by immunoaffinity chromatography using Rabbit IgG coupled to agarose beads.
  • Conjugation notes

    Horseradish Peroxidase (HRP)
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • General notes

    HRP conjugated anti-rabbit secondary antibody optimized for western blot and immunohistochemistry. Some customers reported seeing brown precipitates in the vials. The brown precipitates are very common with HRP conjugated antibodies; we suggest vortexing the vial and using this antibody as normal. Our customer’s feedback says the antibody worked great. If in case the antibody fails to give results then please contact our Scientific Support team for assistance.

    Centrifuge product if not completely clear after standing at room temperature. Dilute only prior to immediate use.
Applications Goat Anti-Rabbit

Applications Goat Anti-Rabbit

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab6721 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

ApplicationAbreviewsNotes
IHC-P(2)
1/1000.
WB(7)

1/2000 – 1/20000.Suggested working dilution of 1/3000 (see PMID: 17222046). In addition, found to work at 1/20000 (see PMID: 16936283).

Working dilutions are highlighted in the table below. Please note that the antibody can be diluted to 1:48,000 to 1:207,000 in many instances.

Read More

ELISA
1/120000.
Immunomicroscopy
Use at an assay dependent concentration.
Dot blot
Use at an assay dependent concentration.
ICC
1/1000 – 1/5000.
IHC-Fr
1/1000.
Properties Goat Anti-Mouse

Properties Goat Anti-Mouse

Goat Anti-Mouse IgG H&L (HRP)

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C. Store In the Dark.
  • Storage buffer

    Preservative: 0.01% Gentamicin sulphate
    Constituents: 0.878% Sodium chloride, 1% BSA, 0.424% Potassium phosphate
  • Concentration
    • 1 mg at 2 mg/ml

     

  • Purity

    Affinity purified
  • Purification notes

    This product was prepared from monospecific antiserum by immunoaffinity chromatography using mouse IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities.
  • Conjugation notes

    Horseradish Peroxidase (HRP)
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • General notes

    The product can contain small precipitates giving a cloudy appearance. However this does not affect the performace of the product. Leaving the product at room temperature in the dark for a few hours may help. Please use as normal and if you do experience problems, contact Scientific Support.
Applications Goat Anti-Mouse

Applications Goat Anti-Mouse

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab6789 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

ApplicationAbreviewsNotes
ICC
1/500 – 1/2500.
IP
Use at an assay dependent concentration. PubMed: 17164758
Dot blot
Use at an assay dependent concentration.
ELISA
1/100000.
IHC-P
1/500 – 1/2500.
IHC-Fr
1/500 – 1/2500.
Immunomicroscopy
Use at an assay dependent concentration.
WB(2)
1/2000 – 1/10000. PubMed: 17413034
Datasheet

Datasheet

Goat Anti-Rabbit IgG H&L (HRP)

Goat Anti-Mouse IgG H&L (HRP)

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