The Q-Extract DNA Extraction PCR Kit is ideal for genotyping. It offers rapid and efficient DNA extraction and amplification of a broad range of mammalian tissues, plant leaves, bird feathers, fish fins and saliva.
DNA extraction from various mammalian tissues and plant leaves in just 8 minutes is followed by PCR amplification by Taq DNA Polymerase 2x Master Mix RED
- Ideal solution for genotyping
- Rapid 8-minute extraction protocol
- Minimal handling
- DNA extracts from mammalian tissues, plant leaves, bacteria, bird feathers and fish fins
- PCR-ready DNA
- Reliable PCR results
- Direct gel loading
- Scalable set-up
The Q-Extract DNA Extraction PCR Kit consists of Q-Extract DNA extraction solution and Taq DNA Polymerase 2x Master Mix RED.
The non-toxic Q-Extract DNA Extraction Solution is optimized for easy extraction of PCR-ready DNA. DNA can be extracted from various mammalian tissues e.g. mouse tails, ear snips, liver, kidney, lung and plant leaves and saliva. The one-step lysis is performed in ether a thermocycler or heating block and is divided into two simple heating steps. The extracted DNA is ready for PCR without further handling such as vortex, centrifugation or dilutions. DNA extraction from fish fins and bird feathers is similar but requires prolonged incubation for optimal lysis.
The extracted DNA is amplified using the user-friendly Taq DNA Polymerase 2x Master Mix RED for PCR.
Taq DNA Polymerase 2x Master Mix RED is a ready-to-use reaction solution. The red dye and stabilizer within the master mix formulation enables the user to load amplified PCR samples directly to the DNA gel. The red dye also provides visualization of pipetting and mixing
The obtained DNA extract is stable at – 20°C for up to one week and at – 80°C for long term storage.
EFFICIENT AMPLIFICATION OF DNA EXTRACTED FROM VARIOUS MAMMALIAN TISSUES
PERFORMANCE OF Q-EXTRACT DNA EXTRACTION KIT COMPARED TO TWO EQUIVALENT PCR KITS FOR GENOTYPING