GC 2x master mixes are ready-to-use mixes based on either GC Buffer I or GC Buffer II. GC TEMPase master mixes are specifically developed for the amplification of GC-rich DNA sequences.
- High success rate with amplification of GC-rich DNA
- High sensitivity, specificity and product yield
- Diminished formation of non-specific products
- Reaction set-up at room temperature
GC-rich 2x master mixes consist of TEMPase Hot Start DNA Polymerase combined with either GC Buffer I or GC Buffer II, dNTPs and MgCl2. Just add DNA template and primers to successfully carry out PCR.
4x GC Buffer I and II are also available separately.
TIP – WHEN TO CHOOSE SPECIALISED GC BUFFERS
If your PCR fails with TEMPase Hot Start Polymerase in combination with Ammonium Buffer, we kindly recommend you to try TEMPase in combination with GC Buffer I either as master mix or GC-rich DNA Target Kit. If your amplification is still not satisfactory, then switch to GC Buffer II.
To save time both GC buffers can be tested at the same time.
OPTIMISATION OF GC-RICH DNA AMPLIFICATION
COVERAGE STUDY OF GC TEMPASE MASTER MIX I