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HomeLife Science Fetal Bovine Serum
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Human Serum from human male AB plasma, USA origin, sterile-filtered Login to see prices
Sigma-Aldrich

Fetal Bovine Serum

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SKU: SIG-F7524-500ML Category: Life Science
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  • Description
  • Properties
  • Datasheet
  • Protocols
Description

Description

Application

FBS is used in a broad range of cell culture applications.

Biochem/physiol Actions

FBS remains a popular media supplement because it provides a wide array of functions in cell culture. FBS delivers nutrients, growth and attachment factors and protects cells from oxidative damage and apoptosis by mechanisms that are difficult to reproduce in serum-free media (SFM) systems. It provides many non-defined growth promoting and survival enhancing factor to cells in culture. Derived from clotted blood, it is the most widely used undefined supplement in eucaryotic, especially mammalian, cell culture. It is typically used with the classical media that were developed in the 1950s and 1960s. These include many popular media such as Minimum Essential Medium (MEM); Dulbecco′s Modified Eagles Media (DME, DMEM); Iscove′s Modified Eagles Medium (IMDM); M199 and Roswell Park Memorial Institute medium, RPMI-1640.

Features and Benefits

F7524 is the same great product as Biochrom GmbH. product S0115.

 

Analysis Note

• Endotoxin and hemoglobin tested

• Tested for the presence of bacteria, virus, and mycoplasma
• Triple filtered with 0.1 micron membrane under aseptic conditions

Legal Information

Not available in USA or Canada. Please check with your local supplier regarding availability and import restrictions in your country.

Properties

Properties

Related CategoriesApoptosis and Cell Cycle,

Cell Biology,

Cell Culture,

Cell Signaling and Neuroscience,

FBS Classic,

Fetal Bovine Serum (FBS) and Other Animal Sera,

Fetal Bovine Serum (FBS) for cell culture,

Other Apoptosis Related Reagents,

Serum

More…

Quality Level

 

400
biological source

 

bovine fetus
sterility

 

sterile-filtered
composition

 

hemoglobin, ≤25 mg/dL
origin

 

non-USA origin
availability

 

not available in USA, Canada
application(s)

 

cell culture | mammalian: suitable
impurities

 

≤10 EU/mL endotoxin
shipped in

 

dry ice
storage temp.

 

−20°C
Datasheet

Datasheet

Product NameFetal Bovine Serum,
non-USA origin, sterile-filtered, suitable for cell culture
Product NumberF7524
Product BrandSIGMA
TESTSPECIFICATION
SourceConfirmed
Appearance (Turbidity)Clear
Appearance (Color)Straw to Amber
Appearance (Form)Liquid
pH6.7 – 8.0
Osmolality (mOsm/kg)260 – 340
SterilityPass
Protein Content30 – 45 g/l
Endotoxin Level<= 10 EU/ml
Hemoglobin<= 25
MycoplasmaNone Detected
PI3None Detected
IBRNone Detected
BVDVTested
Cell Culture TestPass
Cell LineCell Line – Cell Types
Cloning AssayPass
Albumin–
Alpha Globulins–
Beta Globulins–
Gamma Globulin–
IgG–
Protocols

Protocols

Protocols

Cell Culture Protocol 10: Testing Cells for Mycoplasma Contamination by Hoechst DNA Staining

DNA staining methods such as indirect Hoechst staining techniques are quick with results available within 72 hours which compares favorably with 4 weeks for mycoplasma detection by culture isolation….
ECACC Laboratory Handbook 4th Edition
Keywords: Cell culture, Culture media, Detection methods, Indicators, Polymerase chain reaction

Cell Culture Protocol 2: Thawing of Frozen Cell Lines

Many cultures obtained from a culture collection, such as ECACC, will arrive frozen and in order to use the cells they must be thawed and put into culture. It is vital to thaw cells correctly in orde…
ECACC Laboratory Handbook 4th Edition
Keywords: Cell culture, Centrifugation, Culture media

Cell Culture Protocol 4: Subculture of Semi-Adherent Cell Lines

Some cell lines grow as a mixed population (e.g. B95-8 – marmoset) where a proportion of cells do not attach to the tissue culture flask and remain in suspension. Therefore, to maintain this heteroge…
ECACC Laboratory Handbook 4th Edition
Keywords: Cell culture, Culture media

Cell Culture Protocol 5: Subculture of Suspension Cell Lines

In general terms, cell lines derived from blood (e.g. lymphocytes) grow in suspension cultures. Cells may grow as single cells or in clumps (e.g. EBV transformed lymphoblastic cell lines). For these …
ECACC Laboratory Handbook 4th Edition
Keywords: Cell culture, Centrifugation, Culture media, Growth factors

Counting cells using a hemocytometer – Cell Culture Protocol 6

For the majority of manipulations using cell lines, such as transfections, cell fusion techniques, cryopreservation and subculture routines it is necessary to quantify the number of cells prior to us…
ECACC Laboratory Handbook 4th Edition
Keywords: Carcinogens, Cell culture, Cryopreservation, Culture media, Flow cytometry

Subculture of Adherent Cell Lines: Cell Culture Protocol 3

Adherent cell lines will grow in vitro until they have covered the surface area available or the medium is depleted of nutrients. At this point the cell lines should be subcultured or passaged in ord…
ECACC Laboratory Handbook 4th Edition
Keywords: Adhesion, Cell culture, Culture media

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