Overview

With the Guava^® Muse^® cell analyzer, you can achieve highly quantitative results at a fraction of the price, effort, and time. The Muse cell analyzer packs 3-parameter analysis into a compact, easy to use benchtop device, making flow cytometry accessible to anyone, anytime. A user-friendly touchscreen interface, intuitive cell analysis software, and optimized assays work to simplify your research.

Laser-Based Fluorescence Detection

The system delivers high-performance cell analysis using a microcapillary technology and miniaturized optics which occupy one-tenth the space of a typical cytometer. Laser-based fluorescence detection of each cell event can evaluate up to 3 cellular parameters. As a result, the Muse system provides more quantitative results than non-flow based imaging systems, which only examine up to two parameters, are time-consuming, and provide less quantitative data.

Accurate Cell Concentrations

The Muse cell analyzer counts cells more accurately than manual hemocytometry or image-based automated analysis.

Muse cell analysis data was correlated with the theoretical concentration with slope closest to 1, indicating superior accuracy.

Item List

Specification

Accurate Cell Concentrations

The Muse cell analyzer counts cells more accurately than manual hemocytometry or image-based automated analysis.

Muse cell analysis data was correlated with the theoretical concentration with slope closest to 1, indicating superior accuracy.

Precise Results

The Muse cell analyzer counts cells and measures viability more precisely with smaller coefficients of variation (%CV) than manual hemocytometry or image-based automated analysis. Higher %CVs were observed for the Trypan blue-based methods, particularly at lower cell concentrations.

Analysis	Cell Concentration		Visibility
Analysis	Average % CV	% CV Range	Average % CV	% CV Range
Image-based Automated Counter	9.2%	1.2-23.3%	3.7%	0.8-12.1%
Manual Hemocytometer	9.2%	6.3%	4.5%	0.5-9.2%
Muse Cell Analyzer	4.0%	0.3-8.8%	2.2%	0.4-5.6%

Comprehensive Cell Health Measurements

Evaluation of cell health and the potential mechanism of action, is critical is various aspects of drug discovery and cell biology.

Accesories

Muse^® Cell Analyzer: Kits & Reagents

For the assays you rely on most, we’ve developed optimized kits, validated for robust performance on the Muse^® cell analyzer. Typical cell preparation protocols have been condensed and simplified, so sample preparation is fast and easy. You don’t need to optimize any software settings — the Muse instrument calculates all gating parameters and thresholds for you. Spend less time with experimental setup, avoid reagent waste, and save money.

What method do you currently use for fundamental cell counting, cell health, and signaling assays?

Other Cell Analysis Techniques	Muse^® Assays
Hemacytometer: Limited due to low-resolution dye uptake for viability determination Subject to manual counting and calculation errors	Muse^® Count and Viability Assay: Precise, fluorescence-based live/dead cell discrimination Microcapillary fluidics for direct cell counts with industry-leading accuracy
Brightfield Microscopy-Based Automated Hemocytometer/Cell Analyzer: Low accuracy based on small number of cells analyzed, and limited to count and viability measurement	Muse^® Assays: Fluorescent reagents generate signal based on specificity for unique targets Statistical significance achievable from rapid acquisition of thousands of cells
Western Blot and Other Bulk Assays: Population heterogeneity is lost when cells are pooled Methods are time-consuming and require expertise Reagents must be validated and optimized Data is qualitative or semi-quantitative at best	Muse^® Assays: Single-cell data is obtained from every sample Extremely simple ‘pipette only’ assays Validated and optimized for multiple cell types
Large, Open-System Flow Cytometer: Reagents must be validated for application Optimization is time-consuming and uses precious sample Software settings must be determined independently	Muse^® Assays: Fluorescent reagents are chosen for compatibility with lasers and detectors and optimized on multiple cell types Assays have validated, optimized sample preparation protocols and pre-set software settings

Kits & Assays
Product	Category	Part Number
Guava^® SARS-CoV-2 Multi-Antigen Antibody Kit	Protein Detection	FCPA100101
Muse^® Count & Viability Kit 40 mL	Cell Health	MCH100102
Muse^® Count & Viability Kit 240 mL	Cell Health	MCH600103
Muse^® Count & Viability Kit (200X)	Cell Health	MCH100104
Muse^® Annexin V & Dead Cell Kit	Cell Health	MCH100105
Muse^® Cell Cycle Kit	Cell Health	MCH100106
Muse^® Cell Dispersal Reagent	Cell Health	MCH100107
Muse^® Caspase-3/7 Kit	Cell Pathways	MCH100108
Muse^® MultiCaspase Kit	Cell Pathways	MCH100109
Muse^® Autophagy LC3-Antibody Based Kit	Cell Pathways, Autophagy	MCH200109
Muse^® Mitopotential Kit	Cell Health	MCH100110
Muse^® Oxidative Stress Kit	Cell Health	MCH100111
Muse^® Nitric Oxide Kit	Cell Health	MCH100112
Muse^® Ki67 Proliferation Kit	Cell Health	MCH100114
Muse^® Human CD4 T Cell Kit	Immunology	MIM100101
Muse^® Human CD8 T Cell Kit	Immunology	MIM100102
Muse^® Human B Cell Kit	Immunology	MIM100103
Muse^® H2A.X Activation Dual Detection Kit	Cell Pathways	MCH200101
Muse^® EGFR-RTK Activation Dual Detection Kit	Cell Pathways	MCH200102
Muse^® PI3K Activation Dual Detection Assay Kit	Cell Pathways	MCH200103
Muse^® MAPK Activation Dual Detection Assay Kit	Cell Pathways	MCH200104
Muse^® Bcl-2 Activation Dual Detection Assay Kit	Cell Pathways	MCH200105
Muse^® Multi-Color DNA Damage Kit	Cell Pathways	MCH200107
Muse^® PI3K/MAPK Dual Pathway Activation Kit	Cell Pathways	MCH200108
Muse^® Malaria P.f-P.v. Detection Kit	Malaria Research	MPA100101

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Analysis	Cell Concentration		Visibility
Analysis	Average % CV	% CV Range	Average % CV	% CV Range
Image-based Automated Counter	9.2%	1.2-23.3%	3.7%	0.8-12.1%
Manual Hemocytometer	9.2%	6.3%	4.5%	0.5-9.2%
Muse Cell Analyzer	4.0%	0.3-8.8%	2.2%	0.4-5.6%

Other Cell Analysis Techniques	Muse^® Assays
Hemacytometer: Limited due to low-resolution dye uptake for viability determination Subject to manual counting and calculation errors	Muse^® Count and Viability Assay: Precise, fluorescence-based live/dead cell discrimination Microcapillary fluidics for direct cell counts with industry-leading accuracy
Brightfield Microscopy-Based Automated Hemocytometer/Cell Analyzer: Low accuracy based on small number of cells analyzed, and limited to count and viability measurement	Muse^® Assays: Fluorescent reagents generate signal based on specificity for unique targets Statistical significance achievable from rapid acquisition of thousands of cells
Western Blot and Other Bulk Assays: Population heterogeneity is lost when cells are pooled Methods are time-consuming and require expertise Reagents must be validated and optimized Data is qualitative or semi-quantitative at best	Muse^® Assays: Single-cell data is obtained from every sample Extremely simple ‘pipette only’ assays Validated and optimized for multiple cell types
Large, Open-System Flow Cytometer: Reagents must be validated for application Optimization is time-consuming and uses precious sample Software settings must be determined independently	Muse^® Assays: Fluorescent reagents are chosen for compatibility with lasers and detectors and optimized on multiple cell types Assays have validated, optimized sample preparation protocols and pre-set software settings

Kits & Assays
Product	Category	Part Number
Guava^® SARS-CoV-2 Multi-Antigen Antibody Kit	Protein Detection	FCPA100101
Muse^® Count & Viability Kit 40 mL	Cell Health	MCH100102
Muse^® Count & Viability Kit 240 mL	Cell Health	MCH600103
Muse^® Count & Viability Kit (200X)	Cell Health	MCH100104
Muse^® Annexin V & Dead Cell Kit	Cell Health	MCH100105
Muse^® Cell Cycle Kit	Cell Health	MCH100106
Muse^® Cell Dispersal Reagent	Cell Health	MCH100107
Muse^® Caspase-3/7 Kit	Cell Pathways	MCH100108
Muse^® MultiCaspase Kit	Cell Pathways	MCH100109
Muse^® Autophagy LC3-Antibody Based Kit	Cell Pathways, Autophagy	MCH200109
Muse^® Mitopotential Kit	Cell Health	MCH100110
Muse^® Oxidative Stress Kit	Cell Health	MCH100111
Muse^® Nitric Oxide Kit	Cell Health	MCH100112
Muse^® Ki67 Proliferation Kit	Cell Health	MCH100114
Muse^® Human CD4 T Cell Kit	Immunology	MIM100101
Muse^® Human CD8 T Cell Kit	Immunology	MIM100102
Muse^® Human B Cell Kit	Immunology	MIM100103
Muse^® H2A.X Activation Dual Detection Kit	Cell Pathways	MCH200101
Muse^® EGFR-RTK Activation Dual Detection Kit	Cell Pathways	MCH200102
Muse^® PI3K Activation Dual Detection Assay Kit	Cell Pathways	MCH200103
Muse^® MAPK Activation Dual Detection Assay Kit	Cell Pathways	MCH200104
Muse^® Bcl-2 Activation Dual Detection Assay Kit	Cell Pathways	MCH200105
Muse^® Multi-Color DNA Damage Kit	Cell Pathways	MCH200107
Muse^® PI3K/MAPK Dual Pathway Activation Kit	Cell Pathways	MCH200108
Muse^® Malaria P.f-P.v. Detection Kit	Malaria Research	MPA100101

Cytek® Guava® Muse® Cell Analyzer