AQ97 High Fidelity DNA Polymerase 2x Master Mix
AQ97 High Fidelity DNA Polymerase 2x Master Mix is a convenient alternative to AQ97 High Fidelity DNA Polymerase, displaying the following features; high fidelity is measured to >60x Taq DNA Polymerase, fast amplification due to its high extension rate, ability to amplify problematic DNA targets, such as those with low to high GC content and ability to perform amplification on long DNA targets. These features enables accurate and reliable PCR results.
AQ97 High Fidelity DNA Polymerase 2x Master Mix is recommended for applications, which require extremely high fidelity such as cloning/Sub-cloning, NGS applications, SNP analysis and mutagenesis.
- All-in-one 2x master mix for great convenience
- Timesaving reaction set-up
- High fidelity: >60x Taq Fidelity
- 3’→5’ proofreading exonuclease activity
- High elongation rate: 10 sec/kb
- Long-range capability: 11 kb gDNA
- Good coverage on DNA templates with low to high GC content
AQ97 High Fidelity DNA Polymerase 2x Master Mix is a ready to use 2x reaction mix composed of AQ97 High Fidelity DNA Polymerase and an optimised buffer system including dNTPs and magnesium chloride, allowing robust amplification on DNA target with low to high GC content and long DNA targets. The DNA binding domain of this polymerase, ensures excellent high fidelity, long range capacity and also fast amplification results.
AQ97 High Fidelity DNA Polymerase exhibits both 5’→3′ DNA polymerase activity and 3’→5′ proofreading exonuclease activity enabling this polymerase to correct base pair mismatches.
For longer targets than 11 kb we recommend to use AQ97 High Fidelity DNA Polymerase with a capability of amplifying DNA targets up to 18 kb..
For difficult amplicons, such as GC-rich DNA samples, those with complex secondary structures or long amplicons, the addition of 1 – 2 M Betaine Enhancer Solution is recommended.
The fidelity of a polymerase refers to its ability to insert the correct base during PCR. On the contrary, the rate of misincorporation is known as a polymerases error rate. Fidelity depends on the polymerase, the buffer system in use and the quality of your template DNA.
LOW NUMBER OF SUBSTITUTIONS