AQ90 High Fidelity DNA Polymerase 2 U/µl is a proofreading DNA polymerase displaying the following features; high fidelity is measured up to 50x Taq DNA Polymerase, ability to amplify problematic DNA targets, such as those with low to high GC content and ability to perform amplification on long DNA targets. These feature enables accurate and reliable PCR results.
AQ90 High Fidelity DNA Polymerase is recommended for applications, which require extremely high fidelity such as cloning/sub-cloning, NGS applications and mutagenesis.
- High fidelity – measured up to 50x Taq Fidelity
- 3’→5’ proofreading exonuclease activity
- Exceptional low error rate
- Processes up to 8.5 kb gDNA and ≤ 12.5 kb for λDNA
- Good coverage on DNA templates with low to high GC content
AQ90 High Fidelity DNA Polymerase exhibits both 5’→3′ DNA polymerase activity and 3’→5′ proofreading exonuclease activity enabling this polymerase to correct base pair mismatches.
AQ90 High Fidelity DNA Polymerase kit is supported with 10x AQ90 Buffer allowing robust amplification on DNA targets with low to high GC content and long DNA targets. The kit also includes an extra tube of MgCl2 in 25 mM concentration if adjustment of PCR conditions is required.
For difficult amplicons, such as GC-rich DNA samples, those with complex secondary structures or long amplicons, the addition of 1 – 2 M Betaine Enhancer Solution is recommended.
For more convenient handling AQ90 High Fidelity DNA Polymerase is also available as a 2x master mix
The fidelity of a polymerase refers to its ability to insert the correct base during PCR. On the contrary, the rate of misincorporation is known as a polymerases error rate. Fidelity depends on the polymerase, the buffer system in use and the quality of your template DNA.