Ammonium Buffer 15mM MgCl2 (3 x 1.5ml)
Ammonium Buffer is recommended for most PCR applications. It results in high yield of PCR products and minimises the need for optimisation of Mg2+ concentrations or the annealing temperatures.
- Promotes amplification on most DNA templates
- High yield
- High specificity
- Tolerance for primer annealing temperature
- 10x Buffer
AVAILABLE IN 4 FORMULATIONS:
- 15 mM MgCl2,
- Mg2+ free
- Tween free
- Mg2+ + Tween free
Ammonium Buffer promotes high specificity over a broad range of annealing temperatures and Mg2+ concentrations. Ammonium Buffer also works well when dealing with difficult templates, e.g. GC-rich DNA sequences.
TIP – CHOOSE THE RIGHT BUFFER
Mg2+ free buffer is recommended if you need to optimise Mg2+ concentrations in your PCR set-up, especially if your application requires Mg2+ concentration lower than 1.5 mM.
Detergent free buffers are recommended for automation and downstream applications involving fluorescent spectrometry.
PERFORMANCE OF AMPLIQON PCR BUFFER SYSTEM
Examples of PCR amplification of ENG9. TEMPase and the indicated buffers were used at the indicated Mg2+ concentrations or temperatures. The first part shows a Mg2+ dilution series from 0.5 – 4.5 mM MgCl2, at 60°C. The second part shows a temperature gradient from 51 – 66 °C at 1.5 mM MgCl2. M: marker.