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HomeLife ScienceProteomics Abcam Antibody Arrays
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Abcam Antibody Arrays

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Catalogue number: Abcam Antibody Arrays

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Categories: Life Science, Proteomics
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  • Description
Description

ab133993

Cytokine Array – Mouse Cytokine Antibody Array (Membrane, 22 Targets)

ab133994

Cytokine Array – Mouse Cytokine Antibody Array (Membrane, 32 Targets)

ab133995

Cytokine Array – Mouse Cytokine Antibody Array (Membrane, 62 Targets)

ab169810

Cytokine Array – Mouse Cytokine Antibody Array (Membrane, 34 Targets)

 

Sample Type
Cell culture supernatant, Saliva, Milk, Urine, Serum, Plasma, Cell culture extracts, Other biological fluids, Whole Blood, Tissue Extracts, Cell Lysate, Cell culture media
Assay Type
Semi-quantitative
Species Reactivity
Reacts with: Mouse

Product View

ab133993 is for simultaneous detection of 22 Mouse Cytokines. Suitable for all sample types.

Targets: GCSF, GM-CSF, IL-2, IL-3, IL-4, IL-5, IL-6, IL-9, IL-10, IL-12 p40/p70, IL-12p70, IL-13, IL-17, IFN-gamma, MCP-1, MCP-5, RANTES, SCF, sTNF RI, TNF-alpha, Thrombopoietin, VEGF

Cytokine arrays are an antibody-pair-based assay, analogous to ELISA, but using a membrane as a substrate rather than a plate. Capture antibodies are supplied arrayed/spotted on a membrane with each pair of spots representing a different analyte. Sample is added (0.2-1ml of 1 sample to each membrane), and then paired biotinylated detector antibodies and streptavidin HRP. The cytokine array is analyzed using the same methods as a chemiluminescent western blot. Comparison between samples can be by eye or using densitometry software for a semi-quantitative comparison.

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ab133997/ ab133997-4M/ ab133997-8M

Description:
Cytokine Array – Human Cytokine Antibody Array (Membrane, 42 Targets)
Reactivity: Human
Sample type: Cell culture supernatant, Saliva, Milk, Urine, Serum, Plasma, Cell culture extracts, Other biological fluids, Whole Blood, Tissue Extracts, Cell Lysate, Cell culture media
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ab184857
Human GLP1 (7-36) ELISA Kit
Sample Type
Cell culture supernatant, Serum, Cell culture media, Heparin Plasma, Citrate Plasma
Assay Type
Cell culture supernatant, Serum, Cell culture media, Heparin Plasma, Citrate Plasma
Species Reactivity

Reacts with: Human

Predicted to work with: Mouse, Rat, Sheep, Rabbit, Horse

Product View
GLP-1(7-36) in vitro SimpleStep ELISA® (Enzyme-Linked Immunosorbent Assay) kit is designed for the quantitative measurement of GLP-1(7-36) active peptide protein in human serum, plasma and culture supernatants.
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ab197438-22T/ ab197438-50T/ ab197438-8T
Human Cytokine Antibody Array A (40 Targets) – Quantitative
Sample Type
Serum, Plasma, Other biological fluids, Cell Lysate, Cell culture media, Tissue Lysate
Assay Type
Quantitative
Species Reactivity
Reacts with: Human
Product View

ab197438 can be used for quantitative measurement of 40 Human cytokines. Suitable for serum, plasma, cell culture media, other body fluids, cell and tissue lysates.

Targets names: Activin A, AgRP, Angiogenin, ANG-1, Angiostatin, Cathepsin S, CD40, Cripto-1, DAN, DKK-1, E-Cadherin, EpCAM, Fas L, Fcγ RIIB/C, Follistatin, Galectin-7, ICAM-2, IL-13 R1, IL-13R2, IL-17B, IL-2 Rα, IL-2 Rβ, IL-23, LAP, NrCAM, PAI-1, PDGF-AB, Resistin, SDF-1β, sgp130, Shh N, Siglec-5, ST2, TGF-β2, Tie-2, TPO, TRAIL R4, TREM-1, VEGF-C, VEGF R1.

ab197451-50T/ ab197451-8T/ ab197451-22T
Human Inflammation Antibody Array (40 Targets) – Quantitative
Sample Type
Serum, Plasma, Other biological fluids, Cell Lysate, Cell culture media, Tissue Lysate
Assay Type
Quantitative
Species Reactivity
Reacts with: Human
Product View

ab197451 can be used for quantitative measurement of 40 Human Inflammatory Factors. Suitable for serum, plasma, cell culture media, other body fluid, cell and tissue lysates.

Targets names: BLC, Eotaxin, Eotaxin-2, G-CSF, GM-CSF, I-309, ICAM-1, IFNγ, IL-1α, IL-1β, IL-1ra, IL-2, IL-4, IL-5, IL-6, IL-6 sR, IL-7, IL-8, IL-10, IL-11, IL-12p40, IL-12p70, IL-13, IL-15, IL-16, IL-17, MCP-1, M-CSF, MIG, MIP-1α, MIP-1β, MIP-1δ, PDGF-BB, RANTES, TIMP-1, TIMP-2, TNFα, TNFβ, TNF sRI, TNF sRII.

ab197452/ ab197452-8T/ ab197452-22T/ ab197452-50T
Human Ig Isotyping Antibody Array (8 Targets) – Quantitative
Sample Type
Serum, Plasma, Other biological fluids, Cell Lysate, Cell culture media, Tissue Lysate
Assay Type
Quantitative
Species Reactivity
Reacts with: Human
Product View

b197452 can be used for quantitative measurement of 8 Human immunoglobulins. Suitable for blood serum.

Target names: IgA, IgD, IgE, IgM, IgG1, IgG2, IgG3, and IgG4.

Quantitative antibody arrays can be used to quantitate up to 40 cytokines with as little as 50 µL of sample. Arrays are available for 400 human and 200 mouse proteins.

Each glass slide is spotted with 16 identical antibody arrays and is provided with a 16 well gasket to allow separate samples to be applied to each array. Each antibody is spotted in quadruplicate in each array. For reproducible quantitation, eight of the arrays are used with a cocktail of protein standards to produce a standard curve. The same 8 standard curve arrays can be used across multiple slides, allowing measurement of 22 experimental samples with 2 slides, 36 with 3 slides etc.

For high through-put, 4 slides can be nested into a tray matching a standard microplate, allowing automated processing with a liquid handling workstation.

Array processing can be completed within one working day (see workflow diagram below) and arrays can be analyzed with a wide number of laser glass slide array / gene microarray scanners. If you don’t have a suitable scanner then we recommend our membrane antibody arrays, which can be analyzed with any WB chemiluminescent reader.

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ab23468
Anti-GLP1 antibody [11E2]
Description
Mouse monoclonal [11E2] to GLP1
Host Species
Mouse
Specificity
This antibody reacts with all forms of GLP 1, including precursor.
Tested Application
Suitable for: ICC/IF, ELISA, WB
Immunogen
Synthetic peptide corresponding to Human GLP1 aa 98-127. The immunogen sequence corresponds to Glucagon-like peptide 1(7-36), one of the chains formed when cleaved. Immunogen was coupled to a carrier and adsorbed onto aluminum hydroxide gel.
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ab26278
Anti-GLP1 antibody [8G9]
Description
Mouse monoclonal [8G9] to GLP1
Host Species
Mouse
Species Reactivity

Reacts with: Mouse, Rat, Guinea pig, Cow, Human, Pig, Common marmoset

Predicted to work with: a wide range of other species
Tested Application
Suitable for: IHC-P, IHC-Fr, Sandwich ELISA, ELISA
Immunogen
Synthetic peptide corresponding to Human GLP1 aa 7-36. ab26278 reacts with the amidated C-Terminus of GLP-1(7-36) coupled to a carrier.
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ab30679
Anti-GIP antibody [04]
Description
Mouse monoclonal [04] to GIP
Host Species
Mouse
Specificity

Ab30679 recognises GIP. It cross reacts up to 10% with PACAP and GLP2 but <0.1% with glucagon, GLP1 or VIP.

Species Reactivity

Reacts with: Human

Tested Application
Suitable for: IHC-P, ELISA, IHC-Fr
 
Immunogen
Synthetic human GIP coupled to carrier. The immunogen was adsorbed onto Al(OH)3 and emulsified in Freund’s incomplete adjuvant.
______________________________________________________________________________________
ab48134
Anti-GIP antibody
Description
Rabbit polyclonal to GIP
Host Species
Rabbit
Species Reactivity

Reacts with: Human

Immunogen
Synthetic peptide: conjugated to KLH, corresponding to internal sequence amino acids 52-93 of Human GIP. Conjugated to KLH via carboxyl group.

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