ab133993
Cytokine Array – Mouse Cytokine Antibody Array (Membrane, 22 Targets)
ab133994
Cytokine Array – Mouse Cytokine Antibody Array (Membrane, 32 Targets)
ab133995
Cytokine Array – Mouse Cytokine Antibody Array (Membrane, 62 Targets)
ab169810
Cytokine Array – Mouse Cytokine Antibody Array (Membrane, 34 Targets)
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ab133993 is for simultaneous detection of 22 Mouse Cytokines. Suitable for all sample types.
Targets: GCSF, GM-CSF, IL-2, IL-3, IL-4, IL-5, IL-6, IL-9, IL-10, IL-12 p40/p70, IL-12p70, IL-13, IL-17, IFN-gamma, MCP-1, MCP-5, RANTES, SCF, sTNF RI, TNF-alpha, Thrombopoietin, VEGF
Cytokine arrays are an antibody-pair-based assay, analogous to ELISA, but using a membrane as a substrate rather than a plate. Capture antibodies are supplied arrayed/spotted on a membrane with each pair of spots representing a different analyte. Sample is added (0.2-1ml of 1 sample to each membrane), and then paired biotinylated detector antibodies and streptavidin HRP. The cytokine array is analyzed using the same methods as a chemiluminescent western blot. Comparison between samples can be by eye or using densitometry software for a semi-quantitative comparison.
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ab133997/ ab133997-4M/ ab133997-8M
Predicted to work with: Mouse, Rat, Sheep, Rabbit, Horse
ab197438 can be used for quantitative measurement of 40 Human cytokines. Suitable for serum, plasma, cell culture media, other body fluids, cell and tissue lysates.
Targets names: Activin A, AgRP, Angiogenin, ANG-1, Angiostatin, Cathepsin S, CD40, Cripto-1, DAN, DKK-1, E-Cadherin, EpCAM, Fas L, Fcγ RIIB/C, Follistatin, Galectin-7, ICAM-2, IL-13 R1, IL-13R2, IL-17B, IL-2 Rα, IL-2 Rβ, IL-23, LAP, NrCAM, PAI-1, PDGF-AB, Resistin, SDF-1β, sgp130, Shh N, Siglec-5, ST2, TGF-β2, Tie-2, TPO, TRAIL R4, TREM-1, VEGF-C, VEGF R1.
ab197451 can be used for quantitative measurement of 40 Human Inflammatory Factors. Suitable for serum, plasma, cell culture media, other body fluid, cell and tissue lysates.
Targets names: BLC, Eotaxin, Eotaxin-2, G-CSF, GM-CSF, I-309, ICAM-1, IFNγ, IL-1α, IL-1β, IL-1ra, IL-2, IL-4, IL-5, IL-6, IL-6 sR, IL-7, IL-8, IL-10, IL-11, IL-12p40, IL-12p70, IL-13, IL-15, IL-16, IL-17, MCP-1, M-CSF, MIG, MIP-1α, MIP-1β, MIP-1δ, PDGF-BB, RANTES, TIMP-1, TIMP-2, TNFα, TNFβ, TNF sRI, TNF sRII.
Target names: IgA, IgD, IgE, IgM, IgG1, IgG2, IgG3, and IgG4.
Quantitative antibody arrays can be used to quantitate up to 40 cytokines with as little as 50 µL of sample. Arrays are available for 400 human and 200 mouse proteins.
Each glass slide is spotted with 16 identical antibody arrays and is provided with a 16 well gasket to allow separate samples to be applied to each array. Each antibody is spotted in quadruplicate in each array. For reproducible quantitation, eight of the arrays are used with a cocktail of protein standards to produce a standard curve. The same 8 standard curve arrays can be used across multiple slides, allowing measurement of 22 experimental samples with 2 slides, 36 with 3 slides etc.
For high through-put, 4 slides can be nested into a tray matching a standard microplate, allowing automated processing with a liquid handling workstation.
Array processing can be completed within one working day (see workflow diagram below) and arrays can be analyzed with a wide number of laser glass slide array / gene microarray scanners. If you don’t have a suitable scanner then we recommend our membrane antibody arrays, which can be analyzed with any WB chemiluminescent reader.
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Reacts with: Mouse, Rat, Guinea pig, Cow, Human, Pig, Common marmoset
Ab30679 recognises GIP. It cross reacts up to 10% with PACAP and GLP2 but <0.1% with glucagon, GLP1 or VIP.
Reacts with: Human
Reacts with: Human
